Coming back to the problem above, i know that some sandbox account are not connected to anything, their whois request module does not work and they may not recognize your nameservers.
In contrast, E59V had no significant effect on that of PR8 or ca Ann Arbor/60 backbone viruses.
Take together; these data suggest that single change in PA, E59V, is critical for some LAIV vaccines efficient growth in eggs or cell culture.
A good vaccine candidate should grow to high titer in eggs or cell culture.
In this study, through egg adaptation, a LAIV (2 mouse-adapted(ma) Ca/04 H1N1:6WF10att) mounted a higher HA titre in eggs compare to its parental virus.
I also host both ns1 and ns2 on the same box with unique IP for each one.